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验证数据展示

  • WB analysis using 60075-1-Ig

    WB analysis using 60075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of hTERT-RPE cells using 60075-1-Ig

    IF Staining of hTERT-RPE cells using 60075-1-Ig

    IF result of anti-Septin 2 (60075-1-Ig, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 2. Blue: DNA. 1% PFA fixed.

  • WB analysis of HEK-293 using 60075-1-Ig

    WB analysis of HEK-293 using 60075-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at various dilutions incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 60075-1-Ig

    IHC staining of human renal cell carcinoma using 60075-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IHC staining of human renal cell carcinoma using 60075-1-Ig

    IHC staining of human renal cell carcinoma using 60075-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IF Staining of K-562 using 60075-1-Ig

    IF Staining of K-562 using 60075-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HEK-293 using 60075-1-Ig

    WB analysis of HEK-293 using 60075-1-Ig

    WB result of Septin 2 antibody (60075-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells.

  • IF Staining of K-562 using 60075-1-Ig

    IF Staining of K-562 using 60075-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of K-562 using 60075-1-Ig

    FC experiment of K-562 using 60075-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (60075-1-Ig, Clone:2F3B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 60075-1-Ig

    Affinity and Kinetic Characterization of 60075-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 60075-1-Ig against Human SEPT2 were performed. The Affinity Constant is 1.23 nM.

  • Featured Product
  • KD/KO验证

Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 60075-1-Ig

产品说明书

CloneNo. 2F3B2
引用文献(18)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

别名

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells
Positive IHC detected inhuman renal cell carcinoma tissue, human brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells, hTERT-RPE cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60075-1-Ig targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原

CatNo: Ag1946

Product name: Recombinant human SEPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-361 aa of BC014455

Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 2
别名 DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5
计算分子量 41 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC014455
基因名称 Septin 2
Gene ID (NCBI) 4735
RRIDAB_2187016
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15019
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

实验方案

Product Specific Protocols
WB protocol for Septin 2 antibody 60075-1-IgDownload protocol
IHC protocol for Septin 2 antibody 60075-1-IgDownload protocol
IF protocol for Septin 2 antibody 60075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
View Article
humanWB

Cell Res

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Authors - Lei Wang
View Article
humanWB

J Cell Biol

Septins suppress the release of vaccinia virus from infected cells.

Authors - Julia Pfanzelter
View Article
mouseWB,IF

Cell Rep

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Authors - Fernando Calvo
View Article
humanCoIP

iScience

Contribution of septins to human platelet structure and function.

Authors - Oleg V Kim
View Article
canineWB

J Cell Sci

Borg5/Cdc42EP1 restricts contractility and motility in epithelial MDCK cells

Authors - David Cohen
View Article