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验证数据展示

  • WB analysis using 60075-1-Ig (same clone as 60075-1-PBS)

    WB analysis using 60075-1-Ig (same clone as 60075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE cells using 60075-1-Ig (same clone as 60075-1-PBS)

    IF Staining of hTERT-RPE cells using 60075-1-Ig (same clone as 60075-1-PBS)

    IF result of anti-Septin 2 (60075-1-Ig, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 2. Blue: DNA. 1% PFA fixed. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

    WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at various dilutions incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

    IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

    IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

    IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

    WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

    WB result of Septin 2 antibody (60075-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

    IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

    FC experiment of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (60075-1-Ig, Clone:2F3B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50982-1

    Cytometric bead array standard curve of MP50982-1

    Cytometric bead array standard curve of MP50982-1, SEPT2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60075-1-PBS. Detection antibody: 60075-2-PBS. Standard:Ag1946. Range: 0.098-100 ng/mL.

  • Affinity and Kinetic Characterization of 60075-1-Ig

    Affinity and Kinetic Characterization of 60075-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 60075-1-Ig against Human SEPT2 were performed. The Affinity Constant is 1.23 nM.

  • Featured Product
  • KD/KO验证

Septin 2 Monoclonal antibody, PBS Only (Capture)

Septin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 60075-1-PBS

产品说明书

CloneNo. 2F3B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.

产品信息

60075-1-PBS targets Septin 2 as part of a matched antibody pair:

MP50982-1: 60075-1-PBS capture and 60075-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1946

Product name: Recombinant human SEPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-361 aa of BC014455

Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 2
别名 DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5
计算分子量 41 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC014455
基因名称 Septin 2
Gene ID (NCBI) 4735
RRIDAB_2187016
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15019
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)