• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL594-60075

    IF Staining of HepG2 using CL594-60075

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-60075

    IF Staining of MCF-7 using CL594-60075

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • IF Staining of K-562 using CL594-60075

    IF Staining of K-562 using CL594-60075

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for IF/ICC
Cat No. CL594-60075

产品说明书

CloneNo. 2F3B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HepG2 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60075-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60075 targets Septin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1946

Product name: Recombinant human SEPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-361 aa of BC014455

Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 2
别名 NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5
计算分子量 41 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC014455
基因名称 Septin 2
Gene ID (NCBI) 4735
RRIDAB_2883416
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15019
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

实验方案

Product Specific Protocols
IF protocol for CL594 Septin 2 antibody CL594-60075Download protocol
Standard Protocols
Click here to view our Standard Protocols