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验证数据展示

  • IF Staining of HepG2 using CL488-15909

    IF Staining of HepG2 using CL488-15909

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 IDH3A antibody (CL488-15909) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated IDH3A Polyclonal antibody

IDH3A Polyclonal Antibody for IF/ICC
Cat No. CL488-15909

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

IDH3A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IDH3A Ready-To-Use IHC Kit. IDH3A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15909-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15909 targets IDH3A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8662

Product name: Recombinant human IDH3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-366 aa of BC021967

Sequence: MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 3 (NAD+) alpha
别名 IDH3A
计算分子量 366 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC021967
基因名称 IDH3A
Gene ID (NCBI) 3419
RRIDAB_3083978
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50213
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IDH3A antibody CL488-15909Download protocol
Standard Protocols
Click here to view our Standard Protocols