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验证数据展示

  • WB analysis of human skeletal muscle using 15909-1-AP

    WB analysis of human skeletal muscle using 15909-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 15909-1-AP

    IHC staining of human brain using 15909-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with IDH3A antibody included
  • IHC staining of human brain using 15909-1-AP

    IHC staining of human brain using 15909-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with IDH3A antibody included
  • IP experiment of HepG2 using 15909-1-AP

    IP experiment of HepG2 using 15909-1-AP

    IP result of anti-IDH3A (IP:15909-1-AP, 4ug; Detection:15909-1-AP 1:1000) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 15909-1-AP

    IF Staining of HepG2 using 15909-1-AP

    Immunofluorescent analysis of HepG2 cells using 15909-1-AP (IDH3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 15909-1-AP

    WB analysis using 15909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15909-1-AP

    IF Staining of HepG2 using 15909-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3A antibody (15909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 15909-1-AP

    FC experiment of HepG2 using 15909-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IDH3A (15909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

IDH3A Polyclonal antibody

IDH3A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 15909-1-AP

产品说明书

引用文献(24)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

NAD(+)-specific ICDH subunit alpha, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, IDH3, EC:1.1.1.41

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human skeletal muscle tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IDH3A Ready-To-Use IHC Kit. IDH3A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15909-1-AP targets IDH3A in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag8662

Product name: Recombinant human IDH3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-366 aa of BC021967

Sequence: MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 3 (NAD+) alpha
别名 NAD(+)-specific ICDH subunit alpha, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, IDH3, EC:1.1.1.41
计算分子量 366 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC021967
基因名称 IDH3A
Gene ID (NCBI) 3419
RRIDAB_2123282
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50213
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IDH3A antibody 15909-1-APDownload protocol
IHC protocol for IDH3A antibody 15909-1-APDownload protocol
IF protocol for IDH3A antibody 15909-1-APDownload protocol
IP protocol for IDH3A antibody 15909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
View Article
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
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mouseWB

EMBO J

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Authors - Yingjiang Xu
View Article
humanWB,CoIP

Acta Pharmacol Sin

Celastrol inhibits the DPYSL2-JAK/STAT pathway by targeting mito-IDHs mediated mitochondrial metabolism to exhaust breast cancer

Authors - Qiao-Li Shi
View Article
humanIHC

J Invest Dermatol

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

Authors - Zhichao Gu
View Article
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
View Article