Validation Data Gallery
Product Information
GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.
| Description | Immunoprecipitation of GST-fusion proteins and their interacting factors with anti-GST Nanobody conjugated to beads.
• Effectively immunoprecipitates low expressed GST-fusion proteins • Considerably higher affinity than glutathione cellulose and other affinity resins • Strong binding even under harsh washing conditions • No heavy & light chains in your downstream application |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | Glutathione S-Transferase (GST) from Schistosoma japonicum |
| Binding capacity | 7.5-10 μg of recombinant GST per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 nM |
| Compatibility with mass spectrometry | The GST-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631414 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
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| sta_SDS_GST-Trap Agarose (EN) |
| Datasheet |
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| GST-Trap Agarose Datasheet |
| Whitepaper |
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| Immunoprecipitation of GST- and MBP-fusion proteins (PDF) |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (PDF) |
Publications
| Application | Title |
|---|---|
Plant Biotechnol J Wheat yellow mosaic virus NIb targets TaVTC2 to elicit broad-spectrum pathogen resistance in wheat | |
Nat Commun Dephosphorylation of the HIV-1 restriction factor SAMHD1 is mediated by PP2A-B55α holoenzymes during mitotic exit. | |
EMBO J The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis. | |
Curr Biol Aurora B-INCENP Localization at Centromeres/Inner Kinetochores Is Required for Chromosome Bi-orientation in Budding Yeast. | |
J Cell Biol Regulating peroxisome-ER contacts via the ACBD5-VAPB tether by FFAT motif phosphorylation and GSK3β. |