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验证数据展示

  • IF Staining of MCF-7 using CL647-51067

    IF Staining of MCF-7 using CL647-51067

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 Beta Catenin antibody (CL647-51067) at dilution of 1:200.

  • FC experiment of MCF-7 using CL647-51067

    FC experiment of MCF-7 using CL647-51067

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Beta Catenin (CL647-51067) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-51067

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-51067 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin
计算分子量 781 aa, 86 kDa
观测分子量 92 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2920240
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35222
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Beta Catenin antibody CL647-51067Download protocol
Standard Protocols
Click here to view our Standard Protocols