Validation Data Gallery
Product Information
p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.
| Description | The p53-C-term-Trap Agarose Kit contains p53-C-term-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of p53.
• Immunoprecipitation of human and mice p53 isoforms alpha with p53-C-term-Trap • Pull-down of the human p53 proteome with complementary p53-N-term- and p53-C-term-Traps, e.g. p53 isoforms and variants • No heavy & light antibody chains in your downstream application • Short incubation time (60 min at +4°C) • Strong binding even under harsh washing conditions |
| Applications | IP, CoIP |
| Specificity/Target | p53 (isoform alpha); epitope 302-393 aa |
| Tested Reactivity | Human, Mouse |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The p53-C-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631379 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| p53 C-term-Trap Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Publications
| Application | Title |
|---|---|
Am J Respir Crit Care Med Long Noncoding RNA TYKRIL Plays a Role in Pulmonary Hypertension via the p53-mediated Regulation of PDGFRβ. |