验证数据展示
经过测试的应用
| Positive WB detected in | human plasma |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
HRP-66484 targets human IgM in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag1459 Product name: Recombinant human IGHM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC009851 Sequence: FGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant mu |
| 别名 | DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, MU, VH |
| 计算分子量 | 69 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC009851 |
| 基因名称 | IGHM |
| Gene ID (NCBI) | 3507 |
| RRID | AB_2883841 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody detects the heavy chain of human IgM (~75 kDa). It's HRP conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP human IgM antibody HRP-66484 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer | ||
Xenotransplantation An IgM Cleaving Enzyme for Clearance of Anti-Pig Xenoreactive Antibodies in a Nonhuman Primate Model | ||
Xenotransplantation An IgM Cleaving Enzyme for Clearance of Anti-Pig Xenoreactive Antibodies in a Nonhuman Primate Model |