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验证数据展示

  • WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells.. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51134-1

    Cytometric bead array standard curve of MP51134-1

    Cytometric bead array standard curve of MP51134-1, ZAP70 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60200-3-PBS. Detection antibody: 60200-1-PBS. Standard:Ag7943. Range: 0.391-100 ng/mL.

  • Affinity and Kinetic Characterization of 60200-1-Ig

    Affinity and Kinetic Characterization of 60200-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 60200-1-Ig against Human ZAP70 was performed. The affinity constant is 1.38 nM.

  • Affinity and Kinetic Characterization of 60200-1-PBS

    Affinity and Kinetic Characterization of 60200-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 60200-1-Ig against Human ZAP70 was performed. The affinity constant is 1.38 nM..

  • Featured Product
  • KD/KO验证

ZAP70 Monoclonal antibody, PBS Only (Detector)

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 60200-1-PBS

产品说明书

CloneNo. 1A11G9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

1A11G9, 70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60200-1-PBS targets ZAP70 as part of a matched antibody pair:

MP51134-1: 60200-3-PBS capture and 60200-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag7943

Product name: Recombinant human ZAP70 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 149-493 aa of BC039039

Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 zeta-chain (TCR) associated protein kinase 70kDa
别名 1A11G9, 70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC039039
基因名称 ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_10944567
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP43403
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

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