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验证数据展示

  • WB analysis of Jurkat using 60200-1-Ig

    WB analysis of Jurkat using 60200-1-Ig

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells..

  • IF Staining of human tonsillitis using 60200-1-Ig

    IF Staining of human tonsillitis using 60200-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of Jurkat using 60200-1-Ig

    WB analysis of Jurkat using 60200-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 60200-1-Ig

    IHC staining of human lymphoma using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 60200-1-Ig

    IHC staining of human lymphoma using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60200-1-Ig

    IHC staining of human tonsillitis using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 60200-1-Ig

    Affinity and Kinetic Characterization of 60200-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 60200-1-Ig against Human ZAP70 was performed. The affinity constant is 1.38 nM.

  • Featured Product
  • KD/KO验证

ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60200-1-Ig

产品说明书

CloneNo. 1A11G9
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

WB, IHC, IF-P, ELISA

别名

1A11G9, 70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, pig spleen tissue, pig thymus tissue
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

60200-1-Ig targets ZAP70 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, pig
文献引用反应性human, mouse
免疫原

CatNo: Ag7943

Product name: Recombinant human ZAP70 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 149-493 aa of BC039039

Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 zeta-chain (TCR) associated protein kinase 70kDa
别名 1A11G9, 70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC039039
基因名称 ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_10944567
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP43403
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

实验方案

Product Specific Protocols
WB protocol for ZAP70 antibody 60200-1-IgDownload protocol
IHC protocol for ZAP70 antibody 60200-1-IgDownload protocol
IF protocol for ZAP70 antibody 60200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function

Authors - Yuman Wang
View Article
humanWB

Cell Immunol

Cbl-b inhibition promotes less differentiated phenotypes of T cells with enhanced cytokine production

Authors - Junfan Wang
View Article