Validation Data Gallery
![V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound.](/products/pictures/Result-v5tdk.png "V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound.")
Product Information
The ChromoTek V5-Trap® Magnetic Particles M-270 Kit consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
| Description | The V5-Trap Magnetic Particles M-270 Kit contains V5-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of V5-tagged proteins and their interacting factors. V5-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP, Protein purification |
| Specificity/Target | V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein. |
| Binding capacity | 0.62-1.25 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic Particles M-270, size: 2.8 µm high throughput-compatible |
| Elution buffer | V5-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 % Nonidet P40 Substitute, 2 % Triton X-100, 150 mM NaCl, 10 mM TCEP, 10 mM DTT, 50 mM Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 40 nM |
| Compatibility with mass spectrometry | The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2892994 |
| Storage Buffer | PBS with 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| V5-Trap® Magnetic Particles M-270 | 20 reactions (500 µL) |