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验证数据展示

  • IF Staining of human tonsillitis using 67317-1-Ig

    IF Staining of human tonsillitis using 67317-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67317-1-Ig

    IF Staining of human tonsillitis using 67317-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67317-1-Ig

    WB analysis using 67317-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67317-1-Ig

    IHC staining of human tonsillitis using 67317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67317-1-Ig

    IHC staining of human tonsillitis using 67317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TLR8 Monoclonal antibody

TLR8 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67317-1-Ig

产品说明书

CloneNo. 3B6A4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF-P, ELISA

别名

CD288, TLR8, toll like receptor 8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, U-937 cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67317-1-Ig targets TLR8 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag16566

Product name: Recombinant human TLR8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 261-610 aa of BC101075

Sequence: FNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 8
别名 CD288, TLR8, toll like receptor 8
计算分子量 1041 aa, 120 kDa
观测分子量 120 kDa
GenBank蛋白编号BC101075
基因名称 TLR8
Gene ID (NCBI) 51311
RRIDAB_2882577
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NR97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.

实验方案

Product Specific Protocols
WB protocol for TLR8 antibody 67317-1-IgDownload protocol
IHC protocol for TLR8 antibody 67317-1-IgDownload protocol
IF protocol for TLR8 antibody 67317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols