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验证数据展示

  • IF Staining of THP-1 using CL488-66645

    IF Staining of THP-1 using CL488-66645

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 TLR2 antibody (CL488-66645, Clone: 1C1D3 ) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TLR2 Monoclonal antibody

TLR2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66645

产品说明书

CloneNo. 1C1D3

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF/ICC

别名

CD282, TIL4, TLR2, toll like receptor 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 66645-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66645 targets TLR2 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原

CatNo: Ag25626

Product name: Recombinant human TLR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 19-302 aa of BC033756

Sequence: KEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 2
别名 CD282, TIL4, TLR2, toll like receptor 2
计算分子量 784 aa, 90 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC033756
基因名称 TLR2
Gene ID (NCBI) 7097
RRIDAB_3084262
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60603
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TLR2 antibody CL488-66645Download protocol
FC protocol for CL Plus 488 TLR2 antibody CL488-66645Download protocol
Standard Protocols
Click here to view our Standard Protocols