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Validation Data Gallery

  • Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60330 (Biotin-conjugated Vimentin Monoclonal antibody) and HRP-PF00030 (Streptavidin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60330 (Biotin-conjugated Vimentin Monoclonal antibody) and HRP-PF00030 (Streptavidin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using HRP-PF00030 (HRP Streptavidin) at dilution of 1:500 (under 40X lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Primary antibody is Biotin-conjugated Ki-67 Recombinant monoclonal antibody.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using HRP-PF00030 (HRP Streptavidin) at dilution of 1:500 (under 40X lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Primary antibody is Biotin-conjugated Ki-67 Recombinant monoclonal antibody.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using HRP-PF00030 (HRP Streptavidin) at dilution of 1:500 (under 20X lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Primary antibody is Biotin-conjugated Ki-67 Recombinant monoclonal antibody.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using HRP-PF00030 (HRP Streptavidin) at dilution of 1:500 (under 20X lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Primary antibody is Biotin-conjugated Ki-67 Recombinant monoclonal antibody.

  • The antibodies (Biotin-98145-4、Biotin-FcA98138、Biotin-98051) were diluted 5-fold from an initial concentration of 2 ug/mL over 8 points on the plate that had been coated with the antigen, The plate was incubated at 37℃ for 1 hour, wash the plate three times. For the detection, HRP-PE00030: lot#21026977 was used at 1/2000 dilution (100 uL/well), followed by incubation for 1h at 37°C.

    The antibodies (Biotin-98145-4、Biotin-FcA98138、Biotin-98051) were diluted 5-fold from an initial concentration of 2 ug/mL over 8 points on the plate that had been coated with the antigen, The plate was incubated at 37℃ for 1 hour, wash the plate three times. For the detection, HRP-PE00030: lot#21026977 was used at 1/2000 dilution (100 uL/well), followed by incubation for 1h at 37°C.

Streptavidin-HRP Conjugate

Cat No. HRP-PF00030

Applications

ELISA, WB, IHC

Conjugate

HRP

Host

Reactivity

Conjugate:  HRP
规格价格库存

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Product Information

Streptavidin-HRP Conjugate (HRP-PF00030) is the product of conjugating Streptavidin (SA) to Horseradish Peroxidase (HRP). This highly sensitive detector combination can be used for the detection of biotin-labeled antibodies, nucleic acids, proteins, or other biotin-labeled molecules for specific applications including Western, ELISA, IHC, etc. The recommended dilutions of Streptavidin-HRP Conjugate (HRP-PF00030) for various routine applications are shown in the Suggested Dilution Range part, and should be adjusted according to the actual experimental procedures.

Concentration 0.2 mg/mL
Applications ELISA, WB, IHC
Host Species
Species Reactivity
Physical State Liquid
Suggested Dilution Range 1:1000 for western blotting;
1:100 for immunohistochemistry;
1:1000 for ELISA.
Purity
Storage Buffer PBS with 0.1% Proclin 300, pH 7.4.
Storage Instructions 2-8℃ for one year.
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