验证数据展示

WB analysis using 18992-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 18992-1-AP
WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.
IF Staining of mouse eye using 18992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse eye using 18992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 18992-1-AP

产品说明书

引用文献(6)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (6)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse brain tissue, HeLa cells, mouse heart tissue
Positive IHC detected in	mouse brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below

产品信息

18992-1-AP targets SLC24A4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF-P, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	fusion protein 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
别名	Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4
计算分子量	69 kDa
观测分子量	62-90 kDa
GenBank蛋白编号	NM_153646
基因名称	SLC24A4
Gene ID (NCBI)	123041
RRID	AB_10596939
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q8NFF2
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

实验方案

Product Specific Protocols
WB protocol for SLC24A4 antibody 18992-1-AP	Download protocol
IHC protocol for SLC24A4 antibody 18992-1-AP	Download protocol
IF protocol for SLC24A4 antibody 18992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IHC	Bone Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis. Authors - Akane Inoue View Article
rat	WB	J Cell Physiol Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling. Authors - Lacruz Rodrigo S RS View Article
Mouse	IHC,IF	J Dent Res Ameloblast Modulation and Transport of Cl-, Na+, and K+ during Amelogenesis. Authors - A L J J Bronckers View Article
mouse	WB	Calcif Tissue Int Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice. Authors - A L J J Bronckers View Article
human	WB	PeerJ Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma Authors - Mingpeng Jin View Article
mouse	WB	Cells Tissues Organs Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts. Authors - Hu Ping P View Article

验证数据展示

WB analysis using 18992-1-AP

WB analysis of HeLa cells using 18992-1-AP

IF Staining of mouse eye using 18992-1-AP

IF Staining of mouse eye using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.

Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.

Ameloblast Modulation and Transport of Cl-, Na+, and K+ during Amelogenesis.

Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.

Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma

Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.