验证数据展示

IHC staining of human colon cancer using 14875-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14875-1-AP (SCLT1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14875-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14875-1-AP (SCLT1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis using 14875-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 14875-1-AP
IP result of anti-SCLT1 (IP:14875-1-AP, 4ug; Detection:14875-1-AP 1:1000) with human placenta tissue lysate 4000ug.
WB analysis using 14875-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 14875-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF Staining of hTERT-RPE1 using 14875-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green), Centrin 1 antibody (12794-1-AP, orange).
IF Staining of hTERT-RPE1 using 14875-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CEP192 antibody (18832-1-AP, orange), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).
IF Staining of hTERT-RPE1 using 14875-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green), CEP250/CNAP1 antibody (14498-1-AP, orange).

SCLT1 Polyclonal antibody

SCLT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14875-1-AP

产品说明书

引用文献(10)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Sodium channel-associated protein 1, SAP1, hCAP 1A, CAP1A

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (10)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, mouse brain tissue, COLO 320 cells, rat brain tissue, HeLa cells
Positive IP detected in	human placenta tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 5 publications below
IF	See 7 publications below

产品信息

14875-1-AP targets SCLT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	CatNo: Ag6646 Product name: Recombinant human SCLT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-79 aa of BC064428 Sequence: MAAEIDFLREQNRRLNEDFRRYQMESFSKYSSVQKAVCQGEGDDTFENLVFDQSFLAPLVTEYDKHLGELNGQLKYYQA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	sodium channel and clathrin linker 1
别名	Sodium channel-associated protein 1, SAP1, hCAP 1A, CAP1A
计算分子量	81 kDa
观测分子量	36 kDa,75-80 kDa
GenBank蛋白编号	BC064428
基因名称	SCLT1
Gene ID (NCBI)	132320
RRID	AB_2183691
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q96NL6
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for SCLT1 antibody 14875-1-AP	Download protocol
IHC protocol for SCLT1 antibody 14875-1-AP	Download protocol
IF protocol for SCLT1 antibody 14875-1-AP	Download protocol
IP protocol for SCLT1 antibody 14875-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Nat Commun A stress-induced cilium-to-PML-NB route drives senescence initiation Authors - Xiaoyu Ma View Article
human	IF	Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. Authors - Chuan Chen View Article
human	IF	Nat Commun TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating. Authors - Hao Yan View Article
human	IF	Nat Commun Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis. Authors - Qingwen Xu View Article
human	IF	Dev Cell The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Authors - Tomoharu Kanie View Article
mouse	WB	Dev Cell ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia. Authors - Jie Ran View Article

验证数据展示

IHC staining of human colon cancer using 14875-1-AP

IHC staining of human colon cancer using 14875-1-AP

WB analysis using 14875-1-AP

IP experiment of human placenta using 14875-1-AP

WB analysis using 14875-1-AP

WB analysis using 14875-1-AP

IF Staining of hTERT-RPE1 using 14875-1-AP

IF Staining of hTERT-RPE1 using 14875-1-AP

IF Staining of hTERT-RPE1 using 14875-1-AP

SCLT1 Polyclonal antibody

SCLT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

A stress-induced cilium-to-PML-NB route drives senescence initiation

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.