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验证数据展示

  • IF Staining of HeLa using CL488-17146

    IF Staining of HeLa using CL488-17146

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PYCR2 antibody (CL488-17146) at dilution of 1:400.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for IF/ICC
Cat No. CL488-17146

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 17146-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17146 targets PYCR2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10918

Product name: Recombinant human PYCR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC020553

Sequence: MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase family, member 2
别名 FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2
计算分子量 320 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC020553
基因名称 PYCR2
Gene ID (NCBI) 29920
RRIDAB_3083998
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96C36
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PYCR2 antibody CL488-17146Download protocol
Standard Protocols
Click here to view our Standard Protocols