验证数据展示
经过测试的应用
| Positive WB detected in | HeLa, HeLa cells, human testis tissue, HEK-293 cells, Raji cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
产品信息
10519-1-AP targets PCMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0811 Product name: Recombinant human PCMT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC008748 Sequence: MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein-L-isoaspartate (D-aspartate) O-methyltransferase |
| 别名 | L-isoaspartyl protein carboxyl methyltransferase, PCMT-1, PIMT, protein L isoaspartate (D aspartate) O methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-28 kDa |
| GenBank蛋白编号 | BC008748 |
| 基因名称 | PCMT1 |
| Gene ID (NCBI) | 5110 |
| RRID | AB_2160201 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22061 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCMT1 antibody 10519-1-AP | Download protocol |
| IHC protocol for PCMT1 antibody 10519-1-AP | Download protocol |
| IF protocol for PCMT1 antibody 10519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Diabetologia Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase? | ||
Int J Cardiol Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1. | ||
Front Cell Dev Biol LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Transl Cancer Res Elevated expression of protein-L-isoaspartate O-methyltransferase-1 (PCMT1) in cervical cancer | ||
J Exp Clin Cancer Res Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.
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