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验证数据展示

  • WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50479-1

    Cytometric bead array standard curve of MP50479-1

    Cytometric bead array standard curve of MP50479-1, NRP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60067-1-PBS. Detection antibody: 60067-2-PBS. Standard:Ag0931. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO验证

Neuropilin 1/CD304 Monoclonal antibody, PBS Only (Capture)

Neuropilin 1/CD304 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60067-1-PBS

产品说明书

CloneNo. 2H3F6

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rabbit

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Neuropilin 1, NRP1, 2H3F6, BDCA4, CD304

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60067-1-PBS targets Neuropilin 1/CD304 as part of a matched antibody pair:

MP50479-1: 60067-1-PBS capture and 60067-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原

CatNo: Ag0931

Product name: Recombinant human NRP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC007737

Sequence: MERGLPLLCAVLALVLAPAGAFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neuropilin 1
别名 Neuropilin 1, NRP1, 2H3F6, BDCA4, CD304
计算分子量 103 kDa
观测分子量 130 kDa
GenBank蛋白编号BC007737
基因名称 Neuropilin 1
Gene ID (NCBI) 8829
RRIDAB_2150840
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14786
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.