Validation Data Gallery
Product Information
Mdm4/HdmX-Trap Agarose is an affinity resin for IP of Mdm4/HdmX-fusion proteins.
| Description | The Mdm4/HdmX-Trap Agarose Kit contains Mdm4/HdmX-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Mdm4/HdmX and interacting factors.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP |
| Specificity/Target | Double minute 4 protein (Mdm4/HdmX), human Note that binding of Mdm4/HdmX-Trap interferes with binding of p53 to Mdm4 HdmX. |
| Tested Reactivity | Human, Mouse, Hamster |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The Mdm4/HdmX-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631374 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| Mdm4/ HdmX-Trap Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |