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验证数据展示

  • FC experiment of HEK-293 using CL647-66172

    FC experiment of HEK-293 using CL647-66172

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MSH6 (CL647-66172, Clone:2E10B2) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for FC (Intra)
Cat No. CL647-66172

产品说明书

CloneNo. 2E10B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

FC (Intra)

别名

hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MSH6 Ready-To-Use IHC Kit. MSH6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66172-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66172 targets MSH6 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12645

Product name: Recombinant human MSH6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC004246

Sequence: MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 6 (E. coli)
别名 hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein
计算分子量 153 kDa
观测分子量 160 kDa
GenBank蛋白编号BC004246
基因名称 MSH6
Gene ID (NCBI) 2956
RRIDAB_2935044
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP52701
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MSH6 antibody CL647-66172Download protocol
Standard Protocols
Click here to view our Standard Protocols
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