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验证数据展示

  • IF Staining of mouse testis using CL594-15073

    IF Staining of mouse testis using CL594-15073

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-15073) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for IF-P
Cat No. CL594-15073

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

IF-P

别名

EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15073-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-15073 targets METTL3 in IF-P applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag7110

Product name: Recombinant human METTL3 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 229-580 aa of BC001650

Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 3
别名 EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70
计算分子量 64 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC001650
基因名称 METTL3
Gene ID (NCBI) 56339
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86U44
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 METTL3 antibody CL594-15073Download protocol
Standard Protocols
Click here to view our Standard Protocols