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验证数据展示

  • IF Staining of mouse pancreas using CL594-14157

    IF Staining of mouse pancreas using CL594-14157

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

  • IF Staining of mouse pancreas using CL594-14157

    IF Staining of mouse pancreas using CL594-14157

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for IF-P
Cat No. CL594-14157

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

IF-P

别名

Cleavage signal 1 protein, CS 1, CS1, DKFZp313O1039, DKFZp779G0129, FLJ45996, KIAA1927, KRAP, KRAP/SSFA2, SPAG13, sperm specific antigen 2, SSFA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse pancreas tissue
For other applications, we recommend the unconjugated version of this antibody, 14157-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14157 targets KRAP/SSFA2 in IF-P applications and shows reactivity with human, rat, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, rat, mouse
免疫原

CatNo: Ag5339

Product name: Recombinant human KRAP, SSFA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 907-1256 aa of BC028706

Sequence: SAIEMQLRRVLHDIRNSLQNLSQYPMMRGPDPAAAPYSTQKSSVLPLYENTFQELQVMRRSLNLFRTQMMDLELAMLRQQTMVYHHMTEEERFEVDQLQGLRNSVRMELQDLELQLEERLLGLEEQLRAVRMPSPFRSSALMGMCGSRSADNLSCPSPLNVMEPVTELMQEQSYLKSELGLGLGEMGFEIPPGESSESVFSQATSESSSVCSGPSHANRRTGVPSTASVGKSKTPLVARKKVFRASVALTPTAPSRTGSVQTPPDLESSEEVDAAEGAPEVVGPKSEVEEGHGKLPSMPAAEEMHKNVEQDELQQVIREVGMDPISCVILELSMICTGGGVICALEDTCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm specific antigen 2
别名 Cleavage signal 1 protein, CS 1, CS1, DKFZp313O1039, DKFZp779G0129, FLJ45996, KIAA1927, KRAP, KRAP/SSFA2, SPAG13, sperm specific antigen 2, SSFA2
计算分子量 138 kDa
GenBank蛋白编号BC028706
基因名称 SSFA2
Gene ID (NCBI) 6744
RRIDAB_2883405
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28290
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

实验方案

Product Specific Protocols
IF protocol for CL594 KRAP/SSFA2 antibody CL594-14157Download protocol
Standard Protocols
Click here to view our Standard Protocols