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验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-13615

    IF Staining of hTERT-RPE1 using CL488-13615

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT20 antibody (CL488-13615) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for IF/ICC
Cat No. CL488-13615

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, Canine

应用

IF/ICC

别名

hIFT20, IFT20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy IFT20 Ready-To-Use IHC Kit. IFT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13615-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13615 targets IFT20 in IF/ICC applications and shows reactivity with human, mouse, Canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, Canine
免疫原

CatNo: Ag4521

Product name: Recombinant human IFT20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC038094

Sequence: MAKDILGEAGLHFDELNKLRVLDPEVTQQTIELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIFQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 20 homolog (Chlamydomonas)
别名 hIFT20, IFT20
计算分子量 15 kDa
观测分子量 15-18 kDa
GenBank蛋白编号BC038094
基因名称 IFT20
Gene ID (NCBI) 90410
RRIDAB_3672570
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY31
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFT20 antibody CL488-13615Download protocol
Standard Protocols
Click here to view our Standard Protocols