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验证数据展示

  • IF Staining of mouse testis using CL594-17460

    IF Staining of mouse testis using CL594-17460

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-17460

    IF Staining of mouse testis using CL594-17460

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

  • IF Staining of hTERT-RPE1 using CL594-17460

    IF Staining of hTERT-RPE1 using CL594-17460

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL594-17460

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 17460-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17460 targets IFT140 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11119

Product name: Recombinant human IFT140 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1114-1462 aa of BC035577

Sequence: AEDLDETSDPALLARCSDFFIEHSQYERAVELLLAARKYQEALQLCLGQNMSITEEMAEKMTVAKDSSDLPEESRRELLEQIADCCMRQGSYHLATKKYTQAGNKLKAMRALLKSGDTEKITFFASVSRQKEIYIMAANYLQSLDWRKEPEIMKNIIGFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLAKAKAKSPLDQETRLAQLQSRMALVKRFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDVYGFLVEHYVRKEEYQTAYRFLEEMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVPEQVRHNSMEDARELDEEVVEEADDDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 140 homolog (Chlamydomonas)
别名 WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog
计算分子量 1462 aa, 165 kDa
GenBank蛋白编号BC035577
基因名称 IFT140
Gene ID (NCBI) 9742
RRIDAB_2919840
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RY7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

实验方案

Product Specific Protocols
IF protocol for CL594 IFT140 antibody CL594-17460Download protocol
Standard Protocols
Click here to view our Standard Protocols