Validation Data Gallery
![Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.](/products/pictures/Result-otdk.png "Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.")
Product Information
The ChromoTek Halo-Trap Magnetic Particles M-270 Kit consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.
| Description | The Halo-Trap Magnetic Particles M-270 Kit contains Halo-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of Halo-tagged proteins and their interacting factors.
• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc. • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands. |
| Binding capacity | 1.25 μg of recombinant Halo-tag per 25 μL bead slurry |
| Conjugate | Magnetic Particles M-270, size: 2.8 µm high throughput-compatible |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 2 nM |
| Compatibility with mass spectrometry | The Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2894835 |
| Storage Buffer | PBS with 0.09% sodium azide |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Halo-Trap Magnetic Particles M-270 | 20 reactions (500 µL slurry) |