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验证数据展示

  • IF Staining of HEK-293 using CL488-13927

    IF Staining of HEK-293 using CL488-13927

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 GNAQ antibody (CL488-13927) at dilution of 1:200.

  • IF Staining of HEK-293 using CL488-13927

    IF Staining of HEK-293 using CL488-13927

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 GNAQ antibody (CL488-13927) at dilution of 1:50.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GNAQ Polyclonal antibody

GNAQ Polyclonal Antibody for IF/ICC
Cat No. CL488-13927

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q, Guanine nucleotide-binding protein G(q) subunit alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 13927-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13927 targets GNAQ in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4947

Product name: Recombinant human GNAQ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-359 aa of BC057777

Sequence: MTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), q polypeptide
别名 CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q, Guanine nucleotide-binding protein G(q) subunit alpha
计算分子量 42 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC057777
基因名称 GNAQ
Gene ID (NCBI) 2776
RRIDAB_3672580
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50148
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GNAQ antibody CL488-13927Download protocol
Standard Protocols
Click here to view our Standard Protocols