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验证数据展示

  • IF Staining of A549 using CL488-66258

    IF Staining of A549 using CL488-66258

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 FAK antibody (CL488-66258, Clone: 2C5B9 ) at dilution of 1:200, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • IF Staining of A549 using CL488-66258

    IF Staining of A549 using CL488-66258

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 FAK antibody (CL488-66258, Clone: 2C5B9 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FAK Monoclonal antibody

FAK Monoclonal Antibody for IF/ICC
Cat No. CL488-66258

产品说明书

CloneNo. 2C5B9

宿主/亚型

Mouse / IgG2c

种属反应性

human, mouse, rat

应用

IF/ICC

别名

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66258-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66258 targets FAK in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17966

Product name: Recombinant human FAK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 381-678 aa of BC028733

Sequence: ITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTR

种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 PTK2 protein tyrosine kinase 2
别名 FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2
计算分子量 1052 aa, 119 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028733
基因名称 FAK
Gene ID (NCBI) 5747
RRIDAB_2919306
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ05397
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FAK antibody CL488-66258Download protocol
Standard Protocols
Click here to view our Standard Protocols