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验证数据展示

  • IF Staining of HeLa using CL488-18244

    IF Staining of HeLa using CL488-18244

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EIF2AK2, PKR antibody (CL488-18244) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for IF/ICC
Cat No. CL488-18244

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse , rat

应用

IF/ICC

别名

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF2AK2 Ready-To-Use IHC Kit. EIF2AK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18244-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18244 targets EIF2AK2, PKR in IF/ICC applications and shows reactivity with human, mouse , rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse , rat
免疫原

CatNo: Ag13010

Product name: Recombinant human EIF2AK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC093676

Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 2
别名 eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated
计算分子量 551 aa, 62 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC093676
基因名称 EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_3084018
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19525
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF2AK2, PKR antibody CL488-18244Download protocol
Standard Protocols
Click here to view our Standard Protocols