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验证数据展示

  • IF Staining of mouse brain using CL488-13925

    IF Staining of mouse brain using CL488-13925

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCX antibody (CL488-13925) at dilution of 1:50. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated DCX Polyclonal antibody

DCX Polyclonal Antibody for IF-P
Cat No. CL488-13925

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

Lis-X, Lissencephalin-X, Doublin, doublecortin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue

This antibody is not recommended for IHC test.

For other applications, we recommend the unconjugated version of this antibody, 13925-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13925 targets DCX in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4945

Product name: Recombinant human DCX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-360 aa of BC027925

Sequence: MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 doublecortin
别名 Lis-X, Lissencephalin-X, Doublin, doublecortin
计算分子量 402 aa, 45 kDa
观测分子量 40 kDa
GenBank蛋白编号BC027925
基因名称 DCX
Gene ID (NCBI) 1641
RRIDAB_3672579
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43602
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCX antibody CL488-13925Download protocol
Standard Protocols
Click here to view our Standard Protocols