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验证数据展示

  • WB analysis of Raji using 67879-1-Ig (same clone as 67879-1-PBS)

    WB analysis of Raji using 67879-1-Ig (same clone as 67879-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67879-1-Ig (same clone as 67879-1-PBS)

    IHC staining of human breast cancer using 67879-1-Ig (same clone as 67879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAXX antibody included
  • IF Staining of A431 using 67879-1-Ig (same clone as 67879-1-PBS)

    IF Staining of A431 using 67879-1-Ig (same clone as 67879-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAXX antibody (67879-1-Ig, Clone: 1F3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67879-1-PBS in a different storage buffer formulation.

DAXX Monoclonal antibody, PBS Only

DAXX Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67879-1-PBS

产品说明书

CloneNo. 1F3G9

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DAXX Ready-To-Use IHC Kit. DAXX primary antibody included.

产品信息

67879-1-PBS targets DAXX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag14606

Product name: Recombinant human DAXX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC109074

Sequence: MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVD

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 death-domain associated protein
别名 BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx
计算分子量 740 aa, 81 kDa
观测分子量100-130 kDa
GenBank蛋白编号BC109074
基因名称 DAXX
Gene ID (NCBI) 1616
RRIDAB_2918636
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UER7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

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