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验证数据展示

  • WB analysis of Raji using 67879-1-Ig

    WB analysis of Raji using 67879-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67879-1-Ig

    IHC staining of human breast cancer using 67879-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DAXX antibody included
  • IF Staining of A431 using 67879-1-Ig

    IF Staining of A431 using 67879-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAXX antibody (67879-1-Ig, Clone: 1F3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DAXX Monoclonal antibody

DAXX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67879-1-Ig

产品说明书

CloneNo. 1F3G9

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, HeLa cells, Daudi cells, MOLT-4 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy DAXX Ready-To-Use IHC Kit. DAXX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67879-1-Ig targets DAXX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag14606

Product name: Recombinant human DAXX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC109074

Sequence: MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVD

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 death-domain associated protein
别名 BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx
计算分子量 740 aa, 81 kDa
观测分子量100-130 kDa
GenBank蛋白编号BC109074
基因名称 DAXX
Gene ID (NCBI) 1616
RRIDAB_2918636
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UER7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

实验方案

Product Specific Protocols
IF protocol for DAXX antibody 67879-1-IgDownload protocol
IHC protocol for DAXX antibody 67879-1-IgDownload protocol
WB protocol for DAXX antibody 67879-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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