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验证数据展示

  • IF Staining of HeLa using CL555-17516

    IF Staining of HeLa using CL555-17516

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 Cytokeratin 17-Specific antibody (CL555-17516) at dilution of 1:200.

  • FC experiment of HeLa using CL555-17516

    FC experiment of HeLa using CL555-17516

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 17-Specific (CL555-17516) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated Cytokeratin 17-Specific Polyclonal antibody

Cytokeratin 17-Specific Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-17516

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®555
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17516-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-17516 targets Cytokeratin 17-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 17
别名 39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1
计算分子量 48 kDa
GenBank蛋白编号NM_000422
基因名称 Cytokeratin 17
Gene ID (NCBI) 3872
RRIDAB_2919634
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ04695
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

实验方案

Product Specific Protocols
FC protocol for CL555 Cytokeratin 17-Specific antibody CL555-17516Download protocol
IF protocol for CL555 Cytokeratin 17-Specific antibody CL555-17516Download protocol
Standard Protocols
Click here to view our Standard Protocols
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