验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55004-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-55004 targets Cyclin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin B1 |
| 别名 | CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB |
| 计算分子量 | 48 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | NM_031966 |
| 基因名称 | Cyclin B1 |
| Gene ID (NCBI) | 891 |
| RRID | AB_3672852 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14635 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Cyclin B1 antibody CL488-55004 | Download protocol |
| IF protocol for CL Plus 488 Cyclin B1 antibody CL488-55004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
