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验证数据展示

  • IF Staining of NIH/3T3 using CL488-19677

    IF Staining of NIH/3T3 using CL488-19677

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-19677) at dilution of 1:100.

  • IF Staining of HeLa using CL488-19677

    IF Staining of HeLa using CL488-19677

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-19677) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Caspase 3/P17/P19 Polyclonal antibody

Caspase 3/P17/P19 Polyclonal Antibody for IF/ICC
Cat No. CL488-19677

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19677-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19677 targets Caspase 3/P17/P19 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3
计算分子量 32 kDa
观测分子量 32-35 kDa, 17 kDa, 19 kDa
GenBank蛋白编号NM_004346
基因名称 Caspase 3
Gene ID (NCBI) 836
RRIDAB_2919166
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42574
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 3/P17/P19 antibody CL488-19677Download protocol
Standard Protocols
Click here to view our Standard Protocols