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验证数据展示

  • IF Staining of HepG2 using CL647-10852

    IF Staining of HepG2 using CL647-10852

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 CLTA antibody (CL647-10852) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated CLTA Polyclonal antibody

CLTA Polyclonal Antibody for IF/ICC
Cat No. CL647-10852

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLTA Ready-To-Use IHC Kit. CLTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10852-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10852 targets CLTA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1299

Product name: Recombinant human CLTA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-218 aa of BC019287

Sequence: MAELDPFGAPAGAPGGPALGNGVAGAGEEDPAAAFLAQQESEIAGIENDEAFAILDGGAPGPQPHGEPPGGPDAVDGVMNGEYYQESNGPTDSYAAISQVDRLQSEPESIRKWREEQMERLEALDANSRKQEAEWKEKAIKELEEWYARQDEQLQKTKANNRAAEEAFVNDIDESSPGTEWERVARLCDFNPKSSKQAKDVSRMRSVLISLKQAPLVH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clathrin, light chain (Lca)
别名 Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA
计算分子量 27 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC019287
基因名称 CLTA
Gene ID (NCBI) 1211
RRIDAB_2920215
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09496
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CLTA antibody CL647-10852Download protocol
Standard Protocols
Click here to view our Standard Protocols