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验证数据展示

  • FC experiment of MCF-7 using CL647-10122

    FC experiment of MCF-7 using CL647-10122

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CDK2 (CL647-10122) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using CL647-10122

    FC experiment of HeLa using CL647-10122

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated CDK2 Polyclonal antibody (CL647-10122)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for FC (Intra)
Cat No. CL647-10122

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10122-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10122 targets CDK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0166

Product name: Recombinant human CDK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 8-210 aa of BC003065

Sequence: EKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEID

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 2
别名 p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC003065
基因名称 CDK2
Gene ID (NCBI) 1017
RRIDAB_2934807
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24941
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CDK2 antibody CL647-10122Download protocol
Standard Protocols
Click here to view our Standard Protocols