验证数据展示

WB analysis of Hek-293 cells using 10745-1-AP
WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.
WB analysis using 10745-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10745-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
IF Staining of HepG2 using 10745-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of HeLa using 10745-1-AP
IP result of anti-NF-κB p65 (IP:10745-1-AP, 4ug; Detection:10745-1-AP 1:6000) with HeLa cells lysate 1280 ug.
IHC staining of mouse colon using 10745-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
IHC staining of human colon using 10745-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
IF Staining of HT-1080 using 10745-1-AP
Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (10745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Featured Product
KD/KO验证

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10745-1-AP

产品说明书

引用文献(1725)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

NF κB, p65, p65; RELA, RELA, NF Kappa B

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (1725)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells, Raji cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, TNF alpha treated HT-1080 cells

Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 23 publications below
WB	See 1547 publications below
IHC	See 128 publications below
IF	See 271 publications below
IP	See 10 publications below
ELISA	See 1 publications below
CoIP	See 8 publications below
ChIP	See 9 publications below

产品信息

10745-1-AP targets NF-κB p65 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, chIP, ELISA
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, rabbit, monkey, chicken, zebrafish, sheep, goat, fish
免疫原	CatNo: Ag1199 Product name: Recombinant human p65; RELA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC011603 Sequence: MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQ 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	v-rel reticuloendotheliosis viral oncogene homolog A (avian)
别名	NF κB, p65, p65; RELA, RELA, NF Kappa B
计算分子量	65 kDa
观测分子量	65 kDa
GenBank蛋白编号	BC011603
基因名称	NF-κB p65
Gene ID (NCBI)	5970
RRID	AB_2178878
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q04206
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

实验方案

Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-AP	Download protocol
IHC protocol for NF-κB p65 antibody 10745-1-AP	Download protocol
IF protocol for NF-κB p65 antibody 10745-1-AP	Download protocol
IP protocol for NF-κB p65 antibody 10745-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Blood Platelet NLRP6 protects against microvascular thrombosis in sepsis Authors - Huimin Jiang View Article
mouse	WB	Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection Authors - Yumei Que View Article
mouse	IF	Blood PMID: 32291445 Authors - Kunpeng Wu View Article
mouse	WB	Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation Authors - Chuanlong Wang View Article
human	WB	Nat Microbiol IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. Authors - Zhimin Jiang View Article
human	WB	Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression Authors - Xingran Li View Article

验证数据展示

WB analysis of Hek-293 cells using 10745-1-AP

WB analysis using 10745-1-AP

IHC staining of human breast cancer using 10745-1-AP

IF Staining of HepG2 using 10745-1-AP

IP experiment of HeLa using 10745-1-AP

IHC staining of mouse colon using 10745-1-AP

IHC staining of human colon using 10745-1-AP

IF Staining of HT-1080 using 10745-1-AP

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Platelet NLRP6 protects against microvascular thrombosis in sepsis

Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection

PMID: 32291445

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression