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验证数据展示

  • WB analysis of Jurkat using 60200-2-Ig

    WB analysis of Jurkat using 60200-2-Ig

    WB result of ZAP70 antibody (60200-2-Ig, 1:5000) with si-control and si-ZAP70 transfected Jurkat cells..

  • WB analysis of Jurkat using 60200-2-Ig

    WB analysis of Jurkat using 60200-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for WB, ELISA
Cat No. 60200-2-Ig

产品说明书

CloneNo. 3B3C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, ELISA

别名

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60200-2-Ig targets ZAP70 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7943

Product name: Recombinant human ZAP70 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 149-493 aa of BC039039

Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zeta-chain (TCR) associated protein kinase 70kDa
别名 70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC039039
基因名称 ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP43403
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.