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验证数据展示

  • WB analysis of Jurkat using 24744-1-AP

    WB analysis of Jurkat using 24744-1-AP

    WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells..

  • WB analysis using 24744-1-AP

    WB analysis using 24744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 24744-1-AP

    IF Staining of U2OS using 24744-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HeLa using 24744-1-AP

    IP experiment of HeLa using 24744-1-AP

    IP result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:6000) with HeLa cells lysate 1360 ug.

  • IHC staining of mouse brain using 24744-1-AP

    IHC staining of mouse brain using 24744-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24744-1-AP (YTHDF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YTHDF2 antibody included
  • IHC staining of mouse brain using 24744-1-AP

    IHC staining of mouse brain using 24744-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24744-1-AP (YTHDF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YTHDF2 antibody included
  • IF Staining of mouse brain using 24744-1-AP

    IF Staining of mouse brain using 24744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24744-1-AP

    IF Staining of HeLa using 24744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

  • IF Staining of U2OS using 24744-1-AP

    IF Staining of U2OS using 24744-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 24744-1-AP

产品说明书

引用文献(362)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (5)

别名

CLL associated antigen KW 14, CLL-associated antigen KW-14, DF2, HGRG8, NY REN 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inU2OS cells, HeLa cells, sodium arsenite treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24744-1-AP targets YTHDF2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP, RIP, CLIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, chicken, bovine, sheep, goat
免疫原

CatNo: Ag5922

Product name: Recombinant human YTHDF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 339-565 aa of BC002559

Sequence: LSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YTH domain family, member 2
别名 CLL associated antigen KW 14, CLL-associated antigen KW-14, DF2, HGRG8, NY REN 2
计算分子量 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC002559
基因名称 YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2687435
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5A9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity. Due to the high sequence similarity, 24744-1-AP is likely to cross-react with both YTHDF1 and YTHDF3.

实验方案

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-APDownload protocol
IHC protocol for YTHDF2 antibody 24744-1-APDownload protocol
IF protocol for YTHDF2 antibody 24744-1-APDownload protocol
IP protocol for YTHDF2 antibody 24744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

m6A enhances the phase separation potential of mRNA.

Authors - Ryan J Ries
View Article
mouseRIP

Nature

m6A RNA methylation regulates the fate of endogenous retroviruses.

Authors - Tomasz Chelmicki
  • KO Validated
View Article
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
View Article
mouse,humanWB,IF

Nature

Dynamic m(6)A mRNA methylation directs translational control of heat shock response.

Authors - Jun Zhou
View Article
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
View Article
mouse,human

Nat Med

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Authors - Irene Ganan-Gomez
View Article
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