Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.
| Description | The Vimentin-Chromobody visualizes endogenous Vimentin in real time.
• Easily transfect your cells with the Vimentin-Chromobody plasmid • Use Vimentin-Chromobody as a marker of vimentin cytoskeleton • Monitor dynamics of vimentin intermediate filaments in live cells • Vimentin-Chromobody does not affect cell viability • The binding of Vimentin-Chromobody does not influence cell viability or cell motility and thus offers you the unique possibility to non-invasively monitor vimentin cytoskeleton in your cells. |
| Applications | Trace endogenous vimentin in real-time Live cell imaging Monitor epithelial-mesenchymal transition in live cells |
| Specificity | Vimentin intermediate filament protein, tested in human, hamster & dog cells |
| Reporter | TagGFP2 |
| Vector type | Mammalian expression vector |
| Promoter | Constitutive CMV IE |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Sequence | With the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence. |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy |
| Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
| SDS |
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| vcg_SDS_Vimentin-Chromobody® plasmid (TagGFP2) (EN) |
| Datasheet |
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| Vimentin-Chromobody® plasmid (TagGFP) Datasheet |
| EULA |
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| vcg End User License Agreement (EULA) Vimentin-Chromobody TagGFP plasmid non-profit (PDF) |
| vcg End User License Agreement (EULA) Vimentin-Chromobody TagGFP plasmid non-profit (US) (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
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Cancer Res Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology. | |
Methods Cell Biol Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos. |