验证数据展示

WB analysis using 10326-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IF Staining of mouse embryo using 10326-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using TRIM32 antibody (10326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis of mouse colon using 10326-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10326-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of hTERT-RPE1 cells using 10326-1-AP
IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.
IP experiment of U-251 using 10326-1-AP
IP result of anti-TRIM32 (IP:10326-1-AP, 4ug; Detection:10326-1-AP 1:2000) with U-251 cells lysate 1200 ug.
IHC staining of human colon using 10326-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TRIM32 antibody included
IHC staining of human colon using 10326-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TRIM32 antibody included
IF Staining of hTERT-RPE1 using 10326-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TRIM32 antibody (10326-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
FC experiment of A431 using 10326-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TRIM32 (10326-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10326-1-AP

产品说明书

引用文献(15)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (15)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, mouse colon tissue, NIH/3T3 cells, HeLa cells
Positive IP detected in	U-251 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse embryo tissue
Positive IF/ICC detected in	hTERT-RPE1 cells
Positive FC (Intra) detected in	A431 cells

Planning an IHC experiment? We recommend our IHCeasy TRIM32 Ready-To-Use IHC Kit. TRIM32 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:100-1:400
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 9 publications below
WB	See 12 publications below
IHC	See 4 publications below
IF	See 5 publications below
IP	See 1 publications below

产品信息

10326-1-AP targets TRIM32 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag0401 Product name: Recombinant human TRIM32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-430 aa of BC003154 Sequence: FCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLT 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	tripartite motif-containing 32
别名	72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32
计算分子量	72 kDa
观测分子量	70-72 kDa
GenBank蛋白编号	BC003154
基因名称	TRIM32
Gene ID (NCBI)	22954
RRID	AB_2208005
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q13049
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390). Western blot analysis detected TRIM32 at an apparent molecular mass of 70-72 kDa.

实验方案

Product Specific Protocols
WB protocol for TRIM32 antibody 10326-1-AP	Download protocol
IHC protocol for TRIM32 antibody 10326-1-AP	Download protocol
IF protocol for TRIM32 antibody 10326-1-AP	Download protocol
IP protocol for TRIM32 antibody 10326-1-AP	Download protocol
FC protocol for TRIM32 antibody 10326-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Sci Adv An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. Authors - Molly M Wilson View Article
mouse,human	IHC	J Am Soc Nephrol Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis. Authors - Sybille Koehler View Article
mouse	WB	Hum Mol Genet Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Authors - Kudryashova Elena E KO Validated View Article
human	WB	PLoS Pathog TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry Authors - Yifan Xie KD Validated View Article
rat,human	WB,IP,IF	FASEB J Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL. Authors - Lei Liu KD Validated View Article
human	IF	J Cell Sci TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1. Authors - Katrine Stange Overå View Article

验证数据展示

WB analysis using 10326-1-AP

IF Staining of mouse embryo using 10326-1-AP

WB analysis of mouse colon using 10326-1-AP

WB analysis of NIH/3T3 using 10326-1-AP

IF Staining of hTERT-RPE1 cells using 10326-1-AP

IP experiment of U-251 using 10326-1-AP

IHC staining of human colon using 10326-1-AP

IHC staining of human colon using 10326-1-AP

IF Staining of hTERT-RPE1 using 10326-1-AP

FC experiment of A431 using 10326-1-AP

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.