验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13623-1-AP targets TRIM15 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4533 Product name: Recombinant human TRIM15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC038585 Sequence: MPATPSLKVVHELPACTLCAGPLEDAVTIPCGHTFCRLCLPALSQMGAQSSGKILLCPLCQEEEQAETPMAPVPLGPLGETYCEEHGEKIYFFCENDAEFLCVFCREGPTHQAHTVGFLDEAIQPYRDRLRSRLEALSTERDEIEDVKCQEDQKLQVLLTQIESKKHQVETAFERLQQELEQQRCLLLARLRELEQQIWKERDEYITKVSEEVTRLGAQVKELEEKCQQPASELLQDVRVNQSRCEMKTFVSPEAISPDLVKKIRDFHRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 15 |
| 别名 | RING finger protein 93, RNF93, TRIM15, tripartite motif containing 15, Zinc finger protein 178, Zinc finger protein B7, ZNF178, ZNFB7 |
| 计算分子量 | 465 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC038585 |
| 基因名称 | TRIM15 |
| Gene ID (NCBI) | 89870 |
| RRID | AB_2303892 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C019 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM15 antibody 13623-1-AP | Download protocol |
| IHC protocol for TRIM15 antibody 13623-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.
| ||
Biochim Biophys Acta Mol Basis Dis Ubiquitin ligase TRIM15 promotes the progression of pancreatic cancer via the upregulation of the IGF2BP2-TLR4 axis | ||
J Cell Sci TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly.
| ||
iScience Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma | ||
J Virol TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
| ||
J Cancer Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. |
