验证数据展示

WB analysis of THP-1 using 19851-1-AP
WB result of TMEM173/STING antibody (19851-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.
WB analysis of HepG2 using 19851-1-AP
WB result of TMEM173/STING antibody (19851-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and TMEM173/STING knockout HepG2 cells.
WB analysis using 19851-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with STING antibody included
IHC staining of human tonsillitis using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with STING antibody included
IF Staining of human tonsillitis using 19851-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of mouse spleen using 19851-1-AP
IP result of anti-TMEM173/STING (IP:19851-1-AP, 4ug; Detection:19851-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.
IF Staining of HT-29 using 19851-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of THP-1 using 19851-1-AP
1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TMEM173/STING (19851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of mouse brain using 19851-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 19851-1-AP

产品说明书

引用文献(452)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA and More (3)

别名

STING, Endoplasmic reticulum interferon stimulator, ERIS, MITA, MPYS

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (452)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HL-60 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse spleen tissue, Raji cells, THP-1 cells, HT-29 cells, mouse thymus tissue, C2C12 cells, RAW 264.7 cells, mouse testis tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	HT-29 cells, human tonsillitis tissue, THP-1 cells
Positive FC (Intra) detected in	THP-1 cells

Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 50 publications below
WB	See 380 publications below
IHC	See 70 publications below
IF	See 126 publications below
IP	See 13 publications below
CoIP	See 9 publications below

产品信息

19851-1-AP targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, Blocking assay
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, monkey, bovine, hamster
免疫原	CatNo: Ag13921 Product name: Recombinant human TMEM173 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 174-379 aa of BC047779 Sequence: ELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	transmembrane protein 173
别名	STING, Endoplasmic reticulum interferon stimulator, ERIS, MITA, MPYS
计算分子量	379 aa, 42 kDa
观测分子量	35-40 kDa, 80 kDa
GenBank蛋白编号	BC047779
基因名称	STING
Gene ID (NCBI)	340061
RRID	AB_10665370
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q86WV6
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

实验方案

Product Specific Protocols
WB protocol for TMEM173/STING antibody 19851-1-AP	Download protocol
IHC protocol for TMEM173/STING antibody 19851-1-AP	Download protocol
IF protocol for TMEM173/STING antibody 19851-1-AP	Download protocol
IP protocol for TMEM173/STING antibody 19851-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins Authors - Huaqing Deng View Article
mouse	IF	Nature Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C. Authors - Ting-Ting Chu KO Validated View Article
mouse	WB	Nature eccDNAs are apoptotic products with high innate immunostimulatory activity Authors - Yuangao Wang KO Validated View Article
human	WB	Science The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling. Authors - Mena Abdel-Nour View Article
human	WB,IF	Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion Authors - Wenwen Zheng KD Validated View Article
human	WB	Cancer Cell Mutant p53 suppresses innate immune signaling to promote tumorigenesis. Authors - Monisankar Ghosh KD Validated View Article

验证数据展示

WB analysis of THP-1 using 19851-1-AP

WB analysis of HepG2 using 19851-1-AP

WB analysis using 19851-1-AP

IHC staining of human tonsillitis using 19851-1-AP

IHC staining of human tonsillitis using 19851-1-AP

IF Staining of human tonsillitis using 19851-1-AP

IP experiment of mouse spleen using 19851-1-AP

IF Staining of HT-29 using 19851-1-AP

FC experiment of THP-1 using 19851-1-AP

IF Staining of mouse brain using 19851-1-AP

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins

Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.

eccDNAs are apoptotic products with high innate immunostimulatory activity

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Mutant p53 suppresses innate immune signaling to promote tumorigenesis.