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验证数据展示

  • WB analysis using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis using 66350-1-Ig (same clone as 66350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TLR4 Monoclonal antibody, PBS Only

TLR4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66350-1-PBS

产品说明书

CloneNo. 3G9A4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

3G9A4, ARMD10, CD284, hToll, TOLL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66350-1-PBS targets TLR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag13857

Product name: Recombinant human TLR4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 653-839 aa of BC117422

Sequence: KFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 4
别名 3G9A4, ARMD10, CD284, hToll, TOLL
计算分子量 839 aa, 96 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC117422
基因名称 TLR4
Gene ID (NCBI) 7099
RRIDAB_2881730
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00206
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.