验证数据展示

WB analysis using 15193-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD98/SLC3A2 antibody included
IHC staining of mouse testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD98/SLC3A2 antibody included
IHC staining of rat testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD98/SLC3A2 antibody included
IHC staining of rat testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD98/SLC3A2 antibody included
IF Staining of HepG2 using 15193-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO验证

CD98/SLC3A2 Polyclonal antibody

CD98/SLC3A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15193-1-AP

产品说明书

引用文献(60)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

CD98, SLC3A2, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2, MDU1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (60)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, Raji cells, HepG2 cells
Positive IHC detected in	rat testis tissue, mouse testis tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy CD98/SLC3A2 Ready-To-Use IHC Kit. CD98/SLC3A2 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 49 publications below
IHC	See 10 publications below
IF	See 13 publications below
IP	See 1 publications below

产品信息

15193-1-AP targets CD98/SLC3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, bovine, sheep
免疫原	CatNo: Ag7280 Product name: Recombinant human SLC3A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 269-630 aa of BC001061 Sequence: KDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
别名	CD98, SLC3A2, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2, MDU1
计算分子量	68 kDa
观测分子量	85-94 kDa, 120-130 kDa
GenBank蛋白编号	BC001061
基因名称	CD98
Gene ID (NCBI)	6520
RRID	AB_2254909
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P08195
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

实验方案

Product Specific Protocols
WB protocol for CD98/SLC3A2 antibody 15193-1-AP	Download protocol
IHC protocol for CD98/SLC3A2 antibody 15193-1-AP	Download protocol
IF protocol for CD98/SLC3A2 antibody 15193-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	ACS Nano Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy Authors - Rangrang Fan View Article
human	WB,IHC	Sci Transl Med Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain. Authors - Kana Hasegawa View Article
mouse	IF	Nat Commun Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters. Authors - Weiwei Yu View Article
human	IF	J Allergy Clin Immunol Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function. Authors - Siddhartha S Saha View Article
mouse,human	WB,IHC,IF	JACC Basic Transl Sci CircBTBD7-420aa Encoded by hsa_circ_0000563 Regulates the Progression of Atherosclerosis and Construction of circBTBD7-420aa Engineered Exosomes Authors - Xiongkang Gan View Article
human	WB	J Hazard Mater ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes. Authors - Xuan Lai View Article

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验证数据展示

WB analysis using 15193-1-AP

IHC staining of mouse testis using 15193-1-AP

IHC staining of mouse testis using 15193-1-AP

IHC staining of rat testis using 15193-1-AP

IHC staining of rat testis using 15193-1-AP

IF Staining of HepG2 using 15193-1-AP

CD98/SLC3A2 Polyclonal antibody

CD98/SLC3A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy

Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.

Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.

Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function.

CircBTBD7-420aa Encoded by hsa_circ_0000563 Regulates the Progression of Atherosclerosis and Construction of circBTBD7-420aa Engineered Exosomes

ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.