验证数据展示

WB analysis using 26975-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 26975-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.
IF Staining of rat brain using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).
IF Staining of mouse cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of U-87 MG using 26975-1-AP
1X10^6 U-87 MG cells were intracellularly stained with 0.2 ug Anti-Human NeuN (26975-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
IHC staining of mouse cerebellum using 26975-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NeuN antibody included
IHC staining of rat cerebellum using 26975-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NeuN antibody included
IF Staining of mouse brain using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4).

NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Cat No. 26975-1-AP

产品说明书

引用文献(361)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig and More (2)

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA and More (2)

别名

Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (361)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected in	mouse cerebellum tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF-Fro detected in	mouse brain tissue
Positive FC (Intra) detected in	U-87 MG cells

Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:5000-1:30000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 50 publications below
IHC	See 54 publications below
IF	See 288 publications below

产品信息

26975-1-AP targets NeuN in WB, IHC, IF-P, IF-Fro, FC (Intra), Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用	WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
文献引用应用	WB, IHC, IF, Dot blot
经测试反应性	human, mouse, rat, pig
文献引用反应性	human, mouse, rat, pig, monkey, zebrafish
免疫原	CatNo: Ag25689 Product name: Recombinant human NeuN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of NM_001082575 Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	hexaribonucleotide binding protein 3
别名	Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen
观测分子量	46-52 kDa
GenBank蛋白编号	NM_001082575
基因名称	NeuN
Gene ID (NCBI)	146713
RRID	AB_2880708
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	A6NFN3
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).

实验方案

Product Specific Protocols
IF protocol for NeuN antibody 26975-1-AP	Download protocol
IHC protocol for NeuN antibody 26975-1-AP	Download protocol
WB protocol for NeuN antibody 26975-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IHC	Immunity Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury Authors - Yangning Lan View Article
mouse	IF	Brain Behav Immun Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease Authors - Jiaxin Guan View Article
mouse	IF	Adv Sci (Weinh) GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability Authors - Kaiyuan Wang View Article
mouse	IF	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
mouse	IF	Sci Adv CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. Authors - Sefan Asamitsu View Article
human	IF	Cell Death Differ Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4 Authors - Wei Liu View Article

验证数据展示

WB analysis using 26975-1-AP

WB analysis using 26975-1-AP

IF Staining of rat brain using 26975-1-AP

IF Staining of rat cerebellum using 26975-1-AP

IF Staining of mouse cerebellum using 26975-1-AP

IF Staining of mouse cerebellum using 26975-1-AP

FC experiment of U-87 MG using 26975-1-AP

IHC staining of mouse cerebellum using 26975-1-AP

IHC staining of rat cerebellum using 26975-1-AP

IF Staining of mouse brain using 26975-1-AP

NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury

Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease

GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4