验证数据展示

WB analysis using 20854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of SU-DHL-4 using 20854-1-AP
WB result of NSUN2 antibody (20854-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and NSUN2 knockout SU-DHL-4 cells.
WB analysis using 20854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 20854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20854-1-AP
WB result of NSUN2 antibody (20854-1-AP; 1:60000; room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HepG2 cells.
IP experiment of HeLa using 20854-1-AP
IP result of anti-NSUN2 (IP:20854-1-AP, 4ug; Detection:20854-1-AP 1:1000) with HeLa cells lysate 4000ug.
IHC staining of human breast cancer using 20854-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NSUN2 antibody included
IF Staining of A549 using 20854-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 20854-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HeLa using 20854-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NSUN2 (20854-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO验证

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 20854-1-AP

产品说明书

引用文献(89)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

SAKI, RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (89)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HT-1080 cells, HeLa cells, HepG2 cells, SU-DHL-4 cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 35 publications below
WB	See 80 publications below
IHC	See 24 publications below
IF	See 15 publications below
IP	See 6 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

产品信息

20854-1-AP targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, RIP
经测试反应性	human
文献引用反应性	human
免疫原	CatNo: Ag14791 Product name: Recombinant human NSUN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 415-767 aa of BC001041 Sequence: HHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	NOL1/NOP2/Sun domain family, member 2
别名	SAKI, RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203
计算分子量	767 aa, 86 kDa
观测分子量	90-100 kDa
GenBank蛋白编号	BC001041
基因名称	NSUN2
Gene ID (NCBI)	54888
RRID	AB_10693629
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q08J23
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

实验方案

Product Specific Protocols
WB protocol for NSUN2 antibody 20854-1-AP	Download protocol
IHC protocol for NSUN2 antibody 20854-1-AP	Download protocol
IF protocol for NSUN2 antibody 20854-1-AP	Download protocol
IP protocol for NSUN2 antibody 20854-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Signal Transduct Target Ther tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis Authors - Sunyang Ying View Article
human	WB	Nat Biotechnol Identification of direct targets and modified bases of RNA cytosine methyltransferases. Authors - Khoddami Vahid V View Article
mouse	WB	Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Authors - Deniz Simsek View Article
human	WB,IHC	Nat Cell Biol 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs. Authors - Xin Chen View Article
human	WB	Nat Protoc Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Authors - Vahid Khoddami View Article
human	WB	Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach Authors - Liang Lu View Article

验证数据展示

WB analysis using 20854-1-AP

WB analysis of SU-DHL-4 using 20854-1-AP

WB analysis using 20854-1-AP

WB analysis using 20854-1-AP

WB analysis of HepG2 using 20854-1-AP

IP experiment of HeLa using 20854-1-AP

IHC staining of human breast cancer using 20854-1-AP

IF Staining of A549 using 20854-1-AP

IF Staining of HepG2 using 20854-1-AP

FC experiment of HeLa using 20854-1-AP

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis

Identification of direct targets and modified bases of RNA cytosine methyltransferases.

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach