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验证数据展示

  • WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

Mesothelin Monoclonal antibody, PBS Only

Mesothelin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66404-1-PBS

产品说明书

CloneNo. 2E3A4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66404-1-PBS targets Mesothelin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23606

Product name: Recombinant human MSLN protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 273-621 aa of BC003512

Sequence: RSSRDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSVQEALSGTPCLLGPGPVLTVLALLLASTLA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mesothelin
别名 MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor
计算分子量 69 kDa
观测分子量 69 kDa, 40 kDa
GenBank蛋白编号BC003512
基因名称 MSLN
Gene ID (NCBI) 10232
RRIDAB_2881778
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13421
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).